Time prior CPO induction | Group1
| Score | Mortality (%) | Severe reaction (%) |
|---|
| Â | untreated2
| 3.6 | 62.5 | 37.5 |
| Â | TBS | 3.8 | 75 | 25 |
| Â | POPC | 3.9 | 87.5 | 12.5 |
5 min | Â | Â | Â | Â |
| Â | Atropine+2PAM3
| 0.5* | 0 | 0 |
| Â | BL-30504
| 1.3* | 12.5 | 0 |
3 hours | Â | Â | Â | Â |
| Â | Atropine+2PAM3
| 2.1 | 25 | 12.5 |
| Â | BL-30504
| 1.3* | 12.5 | 0 |
| Â | rePON1-TBS4
| 1.4* | 12.5 | 12.5 |
14 hours | Â | Â | Â | Â |
| Â | BL-30504
| 3.0 | 50 | 25 |
| Â | rePON1-TBS4
| 1.8* | 0 | 12.5 |
| Â | rePON1-TBS5
| 1.1* | 12.5 | 12.5 |
- Single oral gavage of CPO (23 mg/kg, 10 mL/kg dose) to male C57BL/6J mice was preceded (5 min, 3 hr or 14 hrs prior to CPO intoxication) by no treatment or by injection of TBS (buffer only), POPC (2.5 mg/animal), rePON1 (0.4 mg and 0.63 mg/animal), BL-3050 (0.4 mg rePON1/animal), and atropine (20 mg/kg) with 2-PAM (25 mg/kg). Animals were observed closely for clinical signs during the first 24 hours following CPO intoxication.
- * - Significant difference (p < 0.05) compared to all control groups using Mann-Whitney test (see Methods and Results and Discussion sections for more details).
- 1 - All groups comprised of 8 mice
- 2 - Mice were only poisoned by CPO
- 3 - Atropine and 2-PAM concentrations were 20 mg/kg and 25 mg/kg, respectively
- 4 - Protein estimation amount for rePON1 was 0.4 mg/animal.
- 5 - Protein estimation amount for rePON1 was 0.63 mg/animal.
