Table 3 Calibration results (Intra Assay Precision)
Analyte | Target Value (ng/ml) MPA (μg/ml) | Measured Value (ng/ml) MPA (μg/ml) | Accuracy (%) | Linear Regression (1/x weighting) |
|---|---|---|---|---|
Cyclosporine A | 0 | < 0 | N/A | y = 0.00419 × + 0.000559 |
23.5 | 24.5 | 104 | r = 0.9996 | |
127 | 121 | 94.9 | ||
299 | 291 | 97.3 | ||
484 | 506 | 105 | ||
703 | 714 | 102 | ||
896 | 877 | 97.8 | ||
Tacrolimus | 0 | < 0 | N/A | y = 0.145 × + 0.00678 |
2.1 | 2.45 | 117 | r = 0.9994 | |
5.8 | 6.06 | 105 | ||
12.1 | 12.3 | 102 | ||
18.1 | 18.7 | 103 | ||
24.4 | 24.2 | 99.3 | ||
42.4 | 41.1 | 97.0 | ||
Sirolimus | 0 | < 0 | N/A | y = 0.104 × + 0.0171 |
2.6 | 2.25 | 86.7 | r = 0.9996 | |
6.6 | 6.21 | 94.2 | ||
12.8 | 13.0 | 102 | ||
20.0 | 20.8 | 104 | ||
29.0 | 28.9 | 99.5 | ||
49.2 | 49.1 | 99.7 | ||
Everolimus | 0 | < 0 | N/A | y = 0.112 × + 9.76e-0.09 |
2.2 | 2.25 | 102 | r = 0.9992 | |
5.9 | 5.66 | 95.8 | ||
11.6 | 11.2 | 96.3 | ||
17.0 | 18.0 | 106 | ||
23.2 | 24.0 | 103 | ||
40.8 | 39.6 | 97 | ||
MPA | 4.27 | 1-point calibration linear through zero | y = 0.00494 x r = 1.0 | |