Figure 1From: Validation of an LC-MS/MS method to determine five immunosuppressants with deuterated internal standards including MPA Column Switching. Pos A: Conditioning of the analytical column and loading of SPE column with aqueous buffer containing 10% methanol. Pos B: Elution from the analytical column (97% methanolic solvent) and rinsing step of precolumn with pure methanol.Back to article page