Paclitaxel | Docetaxel | |||
---|---|---|---|---|
Author | Willey [12] | Andersen et al | Rosing [24] | Andersen et al |
Columns | 1 ml CN-(?) 100 mg# | LRC CN-U 100 mg | CN-E 100 mg | LRC CN-E 100 mg |
Cond 1 | 2 ml MeOH | 10 ml MeOH | 1 ml 0.1% TEA in MeCN | 10 ml MeOH |
Cond 2 | 2 ml AmAc## | 5 ml AmAc | 2 ml MeOH | 2 ml AmAc |
Cond 3 | None | None | 2 ml AmAc | None |
Load | 1 ml 50% plasma* | Up to 8 ml 50% plasma* | 1.1 ml undiluted plasma | 2 ml undiluted plasma |
Wash 1 | 2 ml AmAc | 10 ml AmAc | 2 ml AmAc | 2 ml AmAc |
Wash 2 | 2 ml AmAc/20% MeOH | 1 ml AmAc/20% MeOH | 1 ml water/20% MeOH | 1 ml AmAc/20% MeOH |
Wash 3 | 1 ml hexane | 5 ml hexane | None | 2 ml hexane |
Elution | 2× 1 ml 0.1% TEA in MeCN | 2× 250 μl ethyl acetate | 1× 500 μl 0.1% TEA in MeCN | 2× 250 μl ethyl acetate |
Evap | Nitrogen gas blowdown | Vacuum centrifuge | Nitrogen gas blowdown | Vacuum centrifuge |
Injection volume | 100 μl (of 200 μl) in MeOH/MeCN/AmAc | 90 μl (of 100 μl) in 40% MeCN | 50 μl (of 100 μl) in MeOH/MeCN/water | 90 μl (of 100 μl) in 40% MeCN |